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pvdf membrane for anillin  (Bethyl)


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    Bethyl pvdf membrane for anillin
    Figure 1. <t>Anillin</t> immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).
    Pvdf Membrane For Anillin, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvdf membrane for anillin/product/Bethyl
    Average 90 stars, based on 3 article reviews
    pvdf membrane for anillin - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma"

    Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

    Journal: Oncology Reports

    doi: 10.3892/or_00001051

    Figure 1. Anillin immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).
    Figure Legend Snippet: Figure 1. Anillin immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).

    Techniques Used: Immunostaining, Immunohistochemical staining, Staining, Expressing, Negative Staining, Control

    Figure 2. Anillin is expressed in cytoplasmic and nuclear fractions of the RCC cell line 786-O. Cytosolic (lane 1) and nuclear (lane 2) fractions were isolated and 20 μg of protein was loaded onto the SDS-PAGE gel. Western blotting with anillin antibody staining (upper blot) mainly shows expression in the nuclear fraction, but some expression is present in the cytosolic fraction. The size of anillin protein was identical in both fractions, ~120 kDa (upper blot and data not shown). Lamin A/C expression (lower blot) is present only in the nuclear fraction, excluding contamination of the cyto- solic fraction with nuclear content. Molecular weight markers (in kDa) are shown in the right margin. Representative blots from repeated experiments are shown.
    Figure Legend Snippet: Figure 2. Anillin is expressed in cytoplasmic and nuclear fractions of the RCC cell line 786-O. Cytosolic (lane 1) and nuclear (lane 2) fractions were isolated and 20 μg of protein was loaded onto the SDS-PAGE gel. Western blotting with anillin antibody staining (upper blot) mainly shows expression in the nuclear fraction, but some expression is present in the cytosolic fraction. The size of anillin protein was identical in both fractions, ~120 kDa (upper blot and data not shown). Lamin A/C expression (lower blot) is present only in the nuclear fraction, excluding contamination of the cyto- solic fraction with nuclear content. Molecular weight markers (in kDa) are shown in the right margin. Representative blots from repeated experiments are shown.

    Techniques Used: Isolation, SDS Page, Western Blot, Staining, Expressing, Molecular Weight

    Figure 3. Association between anillin cytoplasmic immunopositivity in RCCs and RCC-specific survival. The number of patients at risk with 0, 5 and 10 year follow-up is shown. The RCC-specific survival was signi- ficantly better among patients with cytoplasmic anillin immunopositive RCC (p=0.03).
    Figure Legend Snippet: Figure 3. Association between anillin cytoplasmic immunopositivity in RCCs and RCC-specific survival. The number of patients at risk with 0, 5 and 10 year follow-up is shown. The RCC-specific survival was signi- ficantly better among patients with cytoplasmic anillin immunopositive RCC (p=0.03).

    Techniques Used:



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    Bethyl pvdf membrane for anillin
    Figure 1. <t>Anillin</t> immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).
    Pvdf Membrane For Anillin, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvdf membrane for anillin/product/Bethyl
    Average 90 stars, based on 1 article reviews
    pvdf membrane for anillin - by Bioz Stars, 2026-03
    90/100 stars
      Buy from Supplier

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    Figure 1. Anillin immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).

    Journal: Oncology Reports

    Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

    doi: 10.3892/or_00001051

    Figure Lengend Snippet: Figure 1. Anillin immunostaining in RCC. Immunohistochemical staining of anillin in RCC samples shows anillin expression in the cytoplasm and nuclei (A, B). Omission of the primary antibody (PBS) served as a negative staining control (C). Magnification x400, scale bar 50 μm (C).

    Article Snippet: After boiling the samples in reducing SDS sample buffer for 5 min, 10 μg of protein was loaded per lane and the samples were electrophoresed on 10% polyacrylamide SDS gel and transferred to a PVDF membrane for anillin (IHC-00298, Bethyl Laboratories) or lamin A/C (NCL-LAM-A/C, Leica Biosystems Newcastle Ltd.) detection.

    Techniques: Immunostaining, Immunohistochemical staining, Staining, Expressing, Negative Staining, Control

    Figure 2. Anillin is expressed in cytoplasmic and nuclear fractions of the RCC cell line 786-O. Cytosolic (lane 1) and nuclear (lane 2) fractions were isolated and 20 μg of protein was loaded onto the SDS-PAGE gel. Western blotting with anillin antibody staining (upper blot) mainly shows expression in the nuclear fraction, but some expression is present in the cytosolic fraction. The size of anillin protein was identical in both fractions, ~120 kDa (upper blot and data not shown). Lamin A/C expression (lower blot) is present only in the nuclear fraction, excluding contamination of the cyto- solic fraction with nuclear content. Molecular weight markers (in kDa) are shown in the right margin. Representative blots from repeated experiments are shown.

    Journal: Oncology Reports

    Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

    doi: 10.3892/or_00001051

    Figure Lengend Snippet: Figure 2. Anillin is expressed in cytoplasmic and nuclear fractions of the RCC cell line 786-O. Cytosolic (lane 1) and nuclear (lane 2) fractions were isolated and 20 μg of protein was loaded onto the SDS-PAGE gel. Western blotting with anillin antibody staining (upper blot) mainly shows expression in the nuclear fraction, but some expression is present in the cytosolic fraction. The size of anillin protein was identical in both fractions, ~120 kDa (upper blot and data not shown). Lamin A/C expression (lower blot) is present only in the nuclear fraction, excluding contamination of the cyto- solic fraction with nuclear content. Molecular weight markers (in kDa) are shown in the right margin. Representative blots from repeated experiments are shown.

    Article Snippet: After boiling the samples in reducing SDS sample buffer for 5 min, 10 μg of protein was loaded per lane and the samples were electrophoresed on 10% polyacrylamide SDS gel and transferred to a PVDF membrane for anillin (IHC-00298, Bethyl Laboratories) or lamin A/C (NCL-LAM-A/C, Leica Biosystems Newcastle Ltd.) detection.

    Techniques: Isolation, SDS Page, Western Blot, Staining, Expressing, Molecular Weight

    Figure 3. Association between anillin cytoplasmic immunopositivity in RCCs and RCC-specific survival. The number of patients at risk with 0, 5 and 10 year follow-up is shown. The RCC-specific survival was signi- ficantly better among patients with cytoplasmic anillin immunopositive RCC (p=0.03).

    Journal: Oncology Reports

    Article Title: Anillin expression is a marker of favourable prognosis in patients with renal cell carcinoma

    doi: 10.3892/or_00001051

    Figure Lengend Snippet: Figure 3. Association between anillin cytoplasmic immunopositivity in RCCs and RCC-specific survival. The number of patients at risk with 0, 5 and 10 year follow-up is shown. The RCC-specific survival was signi- ficantly better among patients with cytoplasmic anillin immunopositive RCC (p=0.03).

    Article Snippet: After boiling the samples in reducing SDS sample buffer for 5 min, 10 μg of protein was loaded per lane and the samples were electrophoresed on 10% polyacrylamide SDS gel and transferred to a PVDF membrane for anillin (IHC-00298, Bethyl Laboratories) or lamin A/C (NCL-LAM-A/C, Leica Biosystems Newcastle Ltd.) detection.

    Techniques: